monoclonal rabbit anti cdk2  (Cell Signaling Technology Inc)

Journal: Cells

Article Title: E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster

doi: 10.3390/cells13181535

Figure Lengend Snippet: Cg CHIP inhibits granulocyte proliferation. ( A ) Representative flow cytometry peak diagrams show the proliferation rate of gated EdU labeling agranulocytes in total agranulocytes. ( B ) The bar graph shows the proliferation rate of agranulocytes ( n = 3). ( C ) Representative flow cytometry peak diagrams showing the proliferation rate of gated EdU labeling granulocytes in total granulocytes. ( D ) The bar graph shows the proliferation rate of granulocytes ( n = 3). * p < 0.05, determined by a two-tailed Student’s t test. ( E ) Schematic of granulocyte isolation for cell cycle and Western blotting analyses. ( F ) The percentage changes of granulocytes in different cell cycle phases. ( G ) The bar graph shows the percentage of agranulocytes in different cell cycle phases ( n = 3). Error bars show mean ± standard deviation ( n = 3). p -values, * p < 0.05, ** p < 0.01, were calculated using a one-way ANOVA with Dunnett’s correction for multiple comparisons. ( H , I ) Protein expression levels of proliferative genes Cg Cyclin B1 and Cg CDK2 in granulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). The data shown are representative of three independent experiments. * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t test.

Article Snippet: The other antibodies used for Western blotting were monoclonal antibodies, including rabbit monoclonal antibodies against PCNA (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit monoclonal antibodies against Cyclin B1 (1:1000, ABclonal, Wuhan, China), rabbit monoclonal antibodies against CDK2 (1:1000, Cell Signaling Technology, Boston, MA, USA), mouse monoclonal antibodies against Histone H3 (1:5000, Proteintech, Chicago, IL, USA), and HRP-conjugated rabbit monoclonal against beta-Tubulin (1:10,000, Proteintech, Chicago, IL, USA).

Techniques: Flow Cytometry, Labeling, Two Tailed Test, Isolation, Western Blot, Standard Deviation, Expressing, Control

Journal: NPJ Precision Oncology

Article Title: Integrative modeling uncovers p21-driven drug resistance and prioritizes therapies for PIK3CA-mutant breast cancer

doi: 10.1038/s41698-024-00496-y

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-CDK2 (clone 78B2) 1:1000 dilution , Cell Signaling Technology , Cat# 2546; RRID:AB_2276129.

Techniques:

Journal: Cell Reports Medicine

Article Title: Co-targeting RANK pathway treats and prevents acquired resistance to CDK4/6 inhibitors in luminal breast cancer

doi: 10.1016/j.xcrm.2023.101120

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti CDK2 (78B2) , Cell Signaling , Cat#2546; RRID:AB_2276129.

Techniques: Recombinant, Control, shRNA, Blocking Assay, Alamar Blue Assay, Flow Cytometry, Isolation, cDNA Synthesis, Bicinchoninic Acid Protein Assay, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Gene Expression, Western Blot, Software